Journal: Frontiers in Nutrition
Article Title: Aurantii Fructus extract alleviates DSS-induced colitis in mice via regulating NF-κB and Nrf2/HO-1 signaling pathways and modulating intestinal microbiota
doi: 10.3389/fnut.2025.1661040
Figure Lengend Snippet: AFE’s effect on the NF-κB pathway in UC mice. AFE inhibits the NF-κB pathway in UC mice. Western blot of protein expression of p-IκBα and IκBα (A,B) , p-p65 and p65 (C,D) in colon tissue. x̄ ± s, n = 3. # p < 0.05, ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group.
Article Snippet: Primary rabbit monoclonal antibodies against p-IκBα (cat. no. bsm-60711R), primary rabbit polyclonal antibodies against IκBα (cat. no. bs-1287R) were purchased from Beijing Bioss Biotechnology Co., Ltd. Primary rabbit monoclonal antibodies against Nrf2 (cat. no. 80593-1-RR), p65 (cat. no. 80979-1-RR), p-p65 (cat. no. 82335-1-RR), primary rabbit polyclonal antibodies against β -actin (cat. no. 81115-1-RR), GAPDH (cat. no. 10494-1-AP), α -Tubulin (cat. no. 11224-1-AP), HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (cat. no. SA00001-2) were purchased from Proteintech Group, Inc. (Wuhan, China).
Techniques: Western Blot, Expressing, Control